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Proceedings Paper

Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery
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Paper Abstract

Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system, which takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intra-cellular structures, being exposed to the laser microbeam, which was not possible using conventional phase-contrast microscopy.

Paper Details

Date Published: 12 February 2009
PDF: 7 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 718210 (12 February 2009); doi: 10.1117/12.809761
Show Author Affiliations
Lingfeng Yu, Univ. of California, Irvine (United States)
Samarendra Mohanty, Univ. of California, Irvine (United States)
Gangjun Liu, Univ. of California, Irvine (United States)
Suzanne Genc, Univ. of California, Irvine (United States)
Myung K. Kim, Univ. of South Florida (United States)
Zhongping Chen, Univ. of California, Irvine (United States)
Michael W. Berns, Univ. of California, Irvine (United States)


Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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