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Proceedings Paper

A high sensitivity multi-spectral three-dimensional fluorescence optical tomography system for small animal imaging
Author(s): Changqing Li; Gregory S. Mitchell; Joyita Dutta; Sangtae Ahn; Richard M. Leahy; Simon R. Cherry
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Paper Abstract

We have designed a three dimensional (3D) fluorescence optical tomography system for small animal imaging based on an innovative system geometry that uses a truncated conical mirror which permits the entire surface of the animal to be viewed simultaneously by a single CCD camera. Compared with traditional approaches that employ a flat mirror, the conical mirror system has approximately 3 times better measurement sensitivity. By utilizing a fast switching filter wheel (switching time < 100 milliseconds), emission data at multiple wavelengths can be efficiently collected. An array of appropriately shaped neutral density filters, mounted on a linear stage, can be used to increase the system measurement dynamic range by 3 orders of magnitude. An x-y galvo mirror scanning system makes it possible to scan a collimated laser beam to any location on the mouse surface. A pattern of structured light incident on the animal surface is used to extract the surface geometry. A finite element based algorithm is applied to model photon propagation in the turbid media and a preconditioned conjugate gradient (PCG) method is used to solve the large linear system matrix. The reconstruction algorithm and the system performance are evaluated by phantom experiments.

Paper Details

Date Published: 12 February 2009
PDF: 8 pages
Proc. SPIE 7174, Optical Tomography and Spectroscopy of Tissue VIII, 717415 (12 February 2009); doi: 10.1117/12.809705
Show Author Affiliations
Changqing Li, Univ. of California, Davis (United States)
Gregory S. Mitchell, Univ. of California, Davis (United States)
Joyita Dutta, Univ. of Southern California (United States)
Sangtae Ahn, Univ. of Southern California (United States)
Richard M. Leahy, Univ. of Southern California (United States)
Simon R. Cherry, Univ. of California, Davis (United States)


Published in SPIE Proceedings Vol. 7174:
Optical Tomography and Spectroscopy of Tissue VIII
Bruce J. Tromberg; Arjun G. Yodh; Mamoru Tamura; Eva M. Sevick-Muraca; Robert R. Alfano, Editor(s)

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