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Proceedings Paper

Intrinsic fluorescent recognition ligand scaffold based on chaperonins and water-soluble semiconductor quantum dots
Author(s): Hongzhi Xie; Basil I. Swanson; Hiromi K. Kawaga; Jonathan D. Trent; Mudalige Kumara; Thomas Ippolito; Mircea Cotlet
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Paper Abstract

We describe genetic engineering of a novel protein-nanoparticle hybrid system with great potential for patterning of various types of nanoparticles and for biosensing applications. The hybrid system is based on a genetically-modified chaperonin protein from the hyperthermophilic archaeon Sulfolobus shibatae. This chaperonin is an 18-subunit double ring, which self-assembles in the presence of Mg ions and ATP. We describe a chaperonin mutant (His-β- loopless:HBLL), with increased access to the central cavity and His-tags on each subunit extending into the central cavity. This mutant binds water-soluble semiconductor quantum dots, creating a protein-encapsulated fluorescent nanoparticle. By adding selective binding sites to the solvent-exposed regions of the chaperonin, this proteinnanoparticle bioconjugate becomes a sensor for specific targets. Using a combination of biochemical and spectroscopic assays, we characterize the formation, stoichiometry, affinity and stability of these novel sensors.

Paper Details

Date Published: 16 February 2009
PDF: 12 pages
Proc. SPIE 7188, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI, 718807 (16 February 2009); doi: 10.1117/12.809576
Show Author Affiliations
Hongzhi Xie, Los Alamos National Lab. (United States)
Basil I. Swanson, Los Alamos National Lab. (United States)
Hiromi K. Kawaga, NASA Ames Research Ctr. (United States)
Jonathan D. Trent, NASA Ames Research Ctr. (United States)
Mudalige Kumara, Brookhaven National Lab. (United States)
Thomas Ippolito, Brookhaven National Lab. (United States)
Mircea Cotlet, Brookhaven National Lab. (United States)

Published in SPIE Proceedings Vol. 7188:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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