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Proceedings Paper

Toward photostable multiplex analyte detection on a single mode planar optical waveguide
Author(s): Harshini Mukundan; Hongzhi Xie; Aaron Anderson; W. Kevin Grace; Jennifer S. Martinez; Basil Swanson
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Paper Abstract

We have developed a waveguide-based optical biosensor for the sensitive and specific detection of biomarkers associated with disease. Our technology combines the superior optical properties of single-mode planar waveguides, the robust nature of functionalized self-assembled monolayer sensing films and the specificity of fluorescence sandwich immunoassays to detect biomarkers in complex biological samples such as serum, urine and sputum. We have previously reported the adaptation of our technology to the detection of biomarkers associated with breast cancer and anthrax. However, these approaches primarily used phospholipid bilayers as the functional film and organic dyes (ex: AlexaFluors) as the fluorescence reporter. Organic dyes are easily photodegraded and are not amenable to multiplexing because of their narrow Stokes' shift. Here we have developed strategies for conjugation of the detector antibodies with quantum dots for use in a multiplex detection platform. We have previously evaluated dihydroxylipoic acid quantum dots for the detection of a breast cancer biomarker. In this manuscript, we investigate the detection of the Bacillus anthracis protective antigen using antibodies conjugated with polymer-coated quantum dots. Kinetics of binding on the waveguide-based biosensor is reported. We compare the sensitivity of quantum dot labeled antibodies to those labeled with AlexaFluor and demonstrate the photostability of the former in our assay platform. In addition, we compare sulfydryl labeling of the antibody in the hinge region to that of nonspecific amine labeling. This is but the first step in developing a multiplex assay for such biomarkers on our waveguide platform.

Paper Details

Date Published: 24 February 2009
PDF: 9 pages
Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 71670A (24 February 2009); doi: 10.1117/12.809530
Show Author Affiliations
Harshini Mukundan, Los Alamos National Lab. (United States)
Hongzhi Xie, Los Alamos National Lab. (United States)
Aaron Anderson, Los Alamos National Lab. (United States)
W. Kevin Grace, Los Alamos National Lab. (United States)
Jennifer S. Martinez, Los Alamos National Lab. (United States)
Basil Swanson, Los Alamos National Lab. (United States)


Published in SPIE Proceedings Vol. 7167:
Frontiers in Pathogen Detection: From Nanosensors to Systems
Philippe M. Fauchet, Editor(s)

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