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Proceedings Paper

Imaging of a targeted PDT drug with fluorescence tomography
Author(s): Dan Muffoletto; Anurag Gupta; Zhiqiang Xu; Chris Mahrer; Gretchen Bauer; Scott Galas; Ravindra K. Pandey; Ulas Sunar
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Paper Abstract

We constructed a whole-body fluorescence tomography instrument to monitor novel bifunctional phototherapeutic drugs (e.g., HPPH-Cyanine dye conjugate) in small animals. The instrument allows dense source and detector sampling with a fast galvo scanner and a CCD detector for improved resolution and sensitivity (Patwardhan et al., 2005). Here we report tissue phantom measurements to evaluate the imaging performance with a newly constructed tomography instrument. Phantom measurements showed that strong fluorescence generated by HPPH-Cyanine dye (HPPH-CD), having high fluorescence quantum yield and long wavelength fluorescence emission, allowed deep tissue imaging. We also report in vivo fluorescence measurements of the conjugate in Nude mice bearing A549 human non-small cell lung carcinoma (NSCLC) tumors at 24 hr post injection to evaluate tumor detection ability of the conjugate. Our results indicate that the HPPH-CD shows preferential uptake in tumors compared to surrounding normal tissue at 24 hr post injection. This study demonstrates a potential use of HPPH-CD in detection (fluorescence imaging) and treatment (PDT) of deeply seated tumors.

Paper Details

Date Published: 18 February 2009
PDF: 6 pages
Proc. SPIE 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII, 716409 (18 February 2009); doi: 10.1117/12.809526
Show Author Affiliations
Dan Muffoletto, Roswell Park Cancer Institute (United States)
Anurag Gupta, Roswell Park Cancer Institute (United States)
Zhiqiang Xu, Roswell Park Cancer Institute (United States)
Chris Mahrer, North Dakota State Univ. (United States)
Gretchen Bauer, Univ. of Rochester (United States)
Scott Galas, Roswell Park Cancer Institute (United States)
Ravindra K. Pandey, Roswell Park Cancer Institute (United States)
Ulas Sunar, Roswell Park Cancer Institute (United States)


Published in SPIE Proceedings Vol. 7164:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII
David H. Kessel, Editor(s)

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