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Proceedings Paper

Optofluidic single molecule flow proteometry
Author(s): Nan Jing; Chao-Kai Chou; Mien-Chie Hung; Jun Kameoka
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Paper Abstract

A microfluidic single molecule fluorescence-based detection scheme is developed to identify target protein direct from cell lysate by using polyclonal antibody. Relative concentration of target protein in solution is determined by twodimensional (2D) photon burst analysis. Compared to conventional ensemble measurement assays, this microfluidic single molecule approach combines the advantages of higher sensitivity, fast processing time, small sample consumption and high resolution quantitative analysis.

Paper Details

Date Published: 5 March 2009
PDF: 4 pages
Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 71850W (5 March 2009); doi: 10.1117/12.809442
Show Author Affiliations
Nan Jing, Texas A&M Univ. (United States)
Chao-Kai Chou, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Mien-Chie Hung, Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Jun Kameoka, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 7185:
Single Molecule Spectroscopy and Imaging II
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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