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Proceedings Paper

Fast and precise detection of ricin with microcapillary sensor system
Author(s): Jun-Tae Lee; Dosi Dosev; Mikaela Nichkova; Zhiya Ma; Shirley Gee; Bruce D. Hammock; Ian M. Kennedy
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Paper Abstract

Ricin is an easily available toxin which can be used as a bio-terror agent. Fast and inexpensive methods for its detection in different samples are needed. Recently we have developed a novel fluorescent sandwich immunoassay for ricin using magnetic-luminescent nanoparticles (MLNPs) as carriers in a microcapillary system for incubation and detection. Antiricin antibody coated MLNPs that were dispersed in buffer solution were introduced in the capillary tube and immobilized inside using an external electromagnet. Then the sample containing ricin was injected while the MLNPs were mixed by an alternating magnetic field. After the incubation, washing solution and secondary antibody conjugated with Alexa-fluorescent were injected into the capillary while the MLNPs were constantly mixed. After the final wash, the particles were immobilized for detection. The total analysis time was reduced to less than forty minutes which is about 8-10 fold improvement in comparison with the plate-based protocols. This system is promising for the development of a portable biosensor and can be used for the detection of other analytes of interest.

Paper Details

Date Published: 23 February 2009
PDF: 9 pages
Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070G (23 February 2009); doi: 10.1117/12.809421
Show Author Affiliations
Jun-Tae Lee, Univ. of California, Davis (United States)
Dosi Dosev, Univ. of California, Davis (United States)
Mikaela Nichkova, Univ. of California, Davis (United States)
Zhiya Ma, Univ. of California, Davis (United States)
Shirley Gee, Univ. of California, Davis (United States)
Bruce D. Hammock, Univ. of California, Davis (United States)
Ian M. Kennedy, Univ. of California, Davis (United States)


Published in SPIE Proceedings Vol. 7207:
Microfluidics, BioMEMS, and Medical Microsystems VII
Wanjun Wang, Editor(s)

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