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Proceedings Paper

Validation of nonlinear interferometric vibrational imaging as a molecular OCT technique by the use of Raman microscopy
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Paper Abstract

We validate a molecular imaging technique called Nonlinear Interferometric Vibrational Imaging (NIVI) by comparing vibrational spectra with those acquired from Raman microscopy. This broadband coherent anti-Stokes Raman scattering (CARS) technique uses heterodyne detection and OCT acquisition and design principles to interfere a CARS signal generated by a sample with a local oscillator signal generated separately by a four-wave mixing process. These are mixed and demodulated by spectral interferometry. Its confocal configuration allows the acquisition of 3D images based on endogenous molecular signatures. Images from both phantom and mammary tissues have been acquired by this instrument and its spectrum is compared with its spontaneous Raman signatures.

Paper Details

Date Published: 19 February 2009
PDF: 6 pages
Proc. SPIE 7168, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIII, 71680T (19 February 2009); doi: 10.1117/12.809384
Show Author Affiliations
Wladimir A. Benalcazar, Beckman Institute for Advanced Science and Technology (United States)
Univ. of Illinois at Urbana-Champaign (United States)
Zhi Jiang, Beckman Institute for Advanced Science and Technology (United States)
Daniel L. Marks, Beckman Institute for Advanced Science and Technology (United States)
Joseph B. Geddes, Beckman Institute for Advanced Science and Technology (United States)
Stephen A. Boppart, Beckman Institute for Advanced Science and Technology (United States)
Univ. of Illinois at Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 7168:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIII
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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