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Proceedings Paper

Spectral polarimetry for assessing cell alignment in cultured tissues
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Paper Abstract

Successful development of cultured tissues is heavily influenced by cell alignment within the tissue scaffold. Proper cell alignment leads to optimum tissue strength. It has been demonstrated that proper alignment is engendered by application of physiologically realistic stresses during the cell proliferation process. In situ monitoring of cell alignment during development thus can provide important feedback information in determining the optimum stresses. T-matrix calculations suggest that cell alignment characteristics (cell aspect and orientation) can be inferred from the spectral polarization of light scattered by the cells. Therefore, a spectral polarimetry system has been created to measure these effects to provide feedback for proper cell alignment. In order to properly use the system, a calibration procedure was first established. The calibration procedure entailed making mathematical predictions for the system performance based on the system components, and then empirically validating these predictions. Upon system calibration, measurements were made on a biologically relevant sample. We present results of experimental measurements on the sample and discuss structural inferences made from these measurements. In addition, we compare our results with structural information obtained from histological analysis.

Paper Details

Date Published: 23 February 2009
PDF: 7 pages
Proc. SPIE 7179, Optics in Tissue Engineering and Regenerative Medicine III, 717909 (23 February 2009); doi: 10.1117/12.809373
Show Author Affiliations
James C. Gladish, Oregon Health & Science Univ. (United States)
Donald D. Duncan, Oregon Health & Science Univ. (United States)

Published in SPIE Proceedings Vol. 7179:
Optics in Tissue Engineering and Regenerative Medicine III
Sean J. Kirkpatrick; Ruikang Wang, Editor(s)

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