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Proceedings Paper

Reflected light imaging of ON and OFF responses in frog retina
Author(s): Xin-Cheng Yao; Lei Liu; Yang-Guo Li
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Paper Abstract

Using a near infrared (NIR) light flood-illumination imager equipped with a high-speed CCD camera, we demonstrated reflected light imaging of stimulus-evoked retinal ON and OFF responses in isolated, but intact, frog eye. Both fast and slow transient intrinsic optical signals (IOSs) were observed. Fast optical response occurred immediately after the stimulus onset, and correlated tightly with the ON and OFF edges of the visible light stimulus. High resolution images revealed both positive (increasing) and negative (decreasing) IOSs, and dynamic optical change at individual CCD pixels could often exceed 10% of the background light intensity. Our experiment on isolated eye suggests that further development of fast, high resolution fundus imager will allow robust detection of fast IOSs in vivo, and thus allow noninvasive, three-dimensional evaluation of retinal neural function.

Paper Details

Date Published: 23 February 2009
PDF: 8 pages
Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 71613I (23 February 2009); doi: 10.1117/12.809371
Show Author Affiliations
Xin-Cheng Yao, The Univ. of Alabama at Birmingham (United States)
Lei Liu, The Univ. of Alabama at Birmingham (United States)
Yang-Guo Li, The Univ. of Alabama at Birmingham (United States)

Published in SPIE Proceedings Vol. 7161:
Photonic Therapeutics and Diagnostics V
Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Kenton W. Gregory M.D.; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Guillermo J. Tearney; Justus F. R. Ilgner; Steen J. Madsen; Laura Marcu; Haishan Zeng, Editor(s)

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