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Proceedings Paper

Intracellular delivery of and sensing with quantum dot bioconjugates
Author(s): James B. Delehanty; Christopher E. Bradburne; Igor L. Medintz; Dorothy Farrell; Thomas Pons; Jeffrey R. Deschamps; Florence M. Brunel; Philip E. Dawson; Hedi Mattoussi
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Paper Abstract

Luminescent semiconductor quantum dots (QDs) possess several unique optical properties that suggest they will be superior reagents compared to traditional organic fluorophores for applications such as the labeling of subcellular structures and the sensing of biological processes within living cells. Chief among these properties are their 1) high quantum yields, 2) broad absorption spectra coupled with narrow symmetric, size-tunable emissions and 3) the ability to excite multiple QD populations at a single wavelength removed from emission. This latter attribute presents the exciting possibility of multiplexed or "multicolor" intracellular imaging and sensing. In order for QDs to reach their full potential as intracellular imaging and sensing reagents, however, facile and robust methodologies for delivering QDs in a controlled manner to specific subcellular locations must be developed. We have investigated a number of strategies to achieve the intracellular delivery of QDs including peptide-mediated, polymer-mediated, and microinjection based methods. In particular, the ability to selectively deliver biofunctionalized QDs to specific intracellular compartments is being targeted. Additionally, long-term QD intracellular QD fate, stability and toxicity are also concomitantly being examined. Cellular delivery experiments utilizing these various schemes will be highlighted and the relative advantages and disadvantages of each approach will be discussed.

Paper Details

Date Published: 3 March 2009
PDF: 8 pages
Proc. SPIE 7189, Colloidal Quantum Dots for Biomedical Applications IV, 71890W (3 March 2009); doi: 10.1117/12.809286
Show Author Affiliations
James B. Delehanty, U.S. Naval Research Lab. (United States)
Christopher E. Bradburne, U.S. Naval Research Lab. (United States)
Igor L. Medintz, U.S. Naval Research Lab. (United States)
Dorothy Farrell, U.S. Naval Research Lab. (United States)
Thomas Pons, U.S. Naval Research Lab. (United States)
Jeffrey R. Deschamps, U.S. Naval Research Lab. (United States)
Florence M. Brunel, The Scripps Research Institute (United States)
Philip E. Dawson, The Scripps Research Institute (United States)
Hedi Mattoussi, U.S. Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 7189:
Colloidal Quantum Dots for Biomedical Applications IV
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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