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Proceedings Paper

Photoactivatable DCDHF fluorophores for single-molecule imaging
Author(s): Samuel J. Lord; Nicholas R. Conley; Hsiao-lu D. Lee; Na Liu; Reichel Samuel; Robert J. Twieg; W. E. Moerner
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Paper Abstract

We have designed and studied the photophysics of a class of organic fluorophores termed "DCDHFs," which were originally used as push-pull chromophores for nonlinear optical applications. In this paper, we describe the general photophysics of many realizations of the DCDHF class of single-molecule emitters. Moreover, we have reengineered a red-emitting DCDHF fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the singlemolecule level in living cells. This molecule and its relatives will provide a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single-molecule emission.

Paper Details

Date Published: 21 February 2009
PDF: 8 pages
Proc. SPIE 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, 719013 (21 February 2009); doi: 10.1117/12.809257
Show Author Affiliations
Samuel J. Lord, Stanford Univ. (United States)
Nicholas R. Conley, Stanford Univ. (United States)
Hsiao-lu D. Lee, Stanford Univ. (United States)
Na Liu, Kent State Univ. (United States)
Reichel Samuel, Kent State Univ. (United States)
Robert J. Twieg, Kent State Univ. (United States)
W. E. Moerner, Stanford Univ. (United States)


Published in SPIE Proceedings Vol. 7190:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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