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Proceedings Paper

Assessment of a new biomimetic scaffold and its effects on bone formation by OCT
Author(s): Ying Yang; Halil M. Aydin; Erhan Piskin; Alicia J El Haj
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Paper Abstract

The ultimate target of bone tissue engineering is to generate functional load bearing bone. By nature, the porous volume in the trabecular bone is occupied by osseous medulla. The natural bone matrix consists of hydroxyapatite (HA) crystals precipitated along the collagen type I fibres. The mineral phase renders bone strength while collagen provides flexibility. Without mineral component, bone is very flexible and can not bear loads, whereas it is brittle in the case of mineral phase without the collagen presence. In this study, we designed and prepared a new type of scaffold which mimics the features of natural bone. The scaffold consists of three different components, a biphasic polymeric base composed of two different biodegradable polymers prepared by using dual porogen approach and bioactive agents, i.e., collagen and HA particles which are distributed throughout the matrix only in the pore surfaces. Interaction of the bioactive scaffolds possessing very high porosity and interconnected pore structures with cells were investigated in a prolonged culture period by using an osteoblastic cell line. The mineral HA particles have a slight different refractive index from the other elements such as polymeric scaffolds and cell/matrix in a tissue engineering constructs, exhibiting brighter images in OCT. Thus, OCT renders a convenient means to assess the morphology and architecture of the blank biomimetic scaffolds. This study also takes a close observation of OCT images for the cultured cell-scaffold constructs in order to assess neo-formed minerals and matrix. The OCT assessments have been compared with the results from confocal and SEM analysis.

Paper Details

Date Published: 18 February 2009
PDF: 6 pages
Proc. SPIE 7166, Optics in Bone Biology and Diagnostics, 71660L (18 February 2009); doi: 10.1117/12.809208
Show Author Affiliations
Ying Yang, Keele Univ. (United Kingdom)
Halil M. Aydin, Hacettepe Univ. and Biyomedtek (Turkey)
Erhan Piskin, Hacettepe Univ. and Biyomedtek (Turkey)
Alicia J El Haj, Keele Univ. (United Kingdom)


Published in SPIE Proceedings Vol. 7166:
Optics in Bone Biology and Diagnostics
Andreas Mandelis, Editor(s)

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