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Proceedings Paper

A multi-spectral dark-field reflectance microscope for tumor margin delineation during breast lumpectomy
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Paper Abstract

Infiltrating neoplastic epithelia induce ultra-structure changes in tissue providing an intrinsic contrast in terms of their local light scattering response. Imaging systems that can enhance this contrast allow for better visualization of tumor boundaries and thus have enormous potential in guiding complex surgical procedures like breast lumpectomy. Highly localized reflectance measurement probes can quantify scattering changes in tissues in situ, but in order to be useful in surgical settings these techniques require an extension to imaging. A novel microsampling reflectance imaging system has been developed to allow rapid quantitative imaging of ultra-structure associated scattering changes in tissues in situ. The imaging system is described in terms of its design, construction and testing for multi-wavelength, telecentric, darkfield illumination and confocal spectroscopic detection, with imaging fields of up to 1.5 cm × 1.5 cm at 100 microns resolution. Spatial confinement of the incident and detected light allows for direct sampling of the scattering spectrum in tissues in situ and the telecentric design ensures consistent sampling of the scattering phase function throughout the entire imaging field. The imaging system was modeled and optimized using the ZEMAX optical design software. Description of the design and results from the optimization process are presented.

Paper Details

Date Published: 24 February 2009
PDF: 6 pages
Proc. SPIE 7187, Biomedical Applications of Light Scattering III, 718710 (24 February 2009); doi: 10.1117/12.809193
Show Author Affiliations
Venkataramanan Krishnaswamy, Dartmouth College (United States)
Wendy A. Wells, Dartmouth-Hitchcock Medical Ctr. (United States)
Ashley M. Laughney, Dartmouth College (United States)
Kimberley S. Samkoe, Dartmouth College (United States)
Brian W. Pogue, Dartmouth College (United States)

Published in SPIE Proceedings Vol. 7187:
Biomedical Applications of Light Scattering III
Adam Wax; Vadim Backman, Editor(s)

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