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Proceedings Paper

High precision spatial and temporal control of neural circuitry using a semi-automated multi-wavelength nanopatterning system
Author(s): Sandhya Mitnala; Michael Huebshman; Christian Herold; Joachim Herz; Harold Garner
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Paper Abstract

It has been one of the most discussed and intriguing topics -the quest to control neural circuitry as a precursor to decoding the operations of the human brain and manipulating its diseased state. Electrophysiology has created a gateway to control this circuitry with high precision. However, it is not practical to apply these techniques to living systems because these techniques are invasive and lack the spatial resolution necessary to properly address various neural cell components, cell assemblies or even tissues. Here we describe a new instrument that has the potential to replace the conventional patch clamping technique, the workhorse of neural physiology. A Digital Light Processing system from Texas Instruments and an Olympus IX71 inverted microscope were combined to achieve neuronal control at a subcellular spatial resolution. Accompanying these two technologies can be almost any light source, and for these experiments a pair of pulsed light sources that produced two pulse trains at different wavelengths tuned to activate or inactivate selectively the ChR2 and NpHR channels that were cloned to express light sensitive versions in neurons. Fura- 2 ratiometric fluorescent dye would be used to read-out calcium activity. The Pulsed light sources and a filter wheel are under computer control using a National Instruments digital control board and a CCD camera used to acquire real time cellular responses to the spatially controlled pulsed light channel activation would be controlled and synchronized using NI LabVIEW software. This will provide for a millisecond precision temporal control of neural circuitry. Thus this technology could provide researchers with an optical tool to control the neural circuitry both spatially and temporally with high precision.

Paper Details

Date Published: 23 February 2009
PDF: 9 pages
Proc. SPIE 7180, Photons and Neurons, 71800X (23 February 2009); doi: 10.1117/12.809171
Show Author Affiliations
Sandhya Mitnala, The Univ. of Texas Southwestern Medical Ctr. at Dallas (United States)
Michael Huebshman, The Univ. of Texas Southwestern Medical Ctr. at Dallas (United States)
Christian Herold, The Univ. of Texas Southwestern Medical Ctr. at Dallas (United States)
Joachim Herz, The Univ. of Texas Southwestern Medical Ctr. at Dallas (United States)
Harold Garner, The Univ. of Texas Southwestern Medical Ctr. at Dallas (United States)


Published in SPIE Proceedings Vol. 7180:
Photons and Neurons
Anita Mahadevan-Jansen; E. Duco Jansen, Editor(s)

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