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Proceedings Paper

Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging
Author(s): R.-Y. He; K.-C. Cho; N.-S. Chang; Y.-D. Su; S.-J. Chen
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Paper Abstract

A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide the fluorescent images of living cell membranes. The proposed microscope with the helps of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having shorter fluorophore lifetime. In comparison with one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. Combining with the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated the brighter and more contrast fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

Paper Details

Date Published: 13 February 2009
PDF: 9 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831L (13 February 2009); doi: 10.1117/12.809134
Show Author Affiliations
R.-Y. He, National Cheng Kung Univ. (Taiwan)
K.-C. Cho, National Cheng Kung Univ. (Taiwan)
N.-S. Chang, National Cheng Kung Univ. (Taiwan)
Y.-D. Su, National Cheng Kung Univ. (Taiwan)
S.-J. Chen, National Cheng Kung Univ. (Taiwan)

Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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