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Proceedings Paper

Observation of living cells with gold nanoparticles by using surface-enhanced Raman scattering
Author(s): Katsumasa Fujita; Jun Ando; Sawako Ishitobi; Keisaku Hamada; Nicholas I. Smith; Yasushi Inouye; Satoshi Kawata
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Paper Abstract

We observed spatial and temporal behaviors of surface enhanced Raman scattering (SERS) signals with gold nanoparticles in living cells. Gold nanoparticles with the diameter of 50 nm were introduced into macrophage cells through endocytosis. We performed observation of SERS signals from a macrophage with 785 nm excitation. Strong SERS signal from the particles in the cell was observed, and spectrum from each particle shows difference in Raman peaks and intensity. We also observed time-lapse SERS and dark-field image with a frame rate of 3 min. We confirmed that strong SERS signal from the particle in the cell and the spectral changes with positions of the particles in the cell.

Paper Details

Date Published: 18 February 2009
PDF: 5 pages
Proc. SPIE 7192, Plasmonics in Biology and Medicine VI, 71920Q (18 February 2009); doi: 10.1117/12.809115
Show Author Affiliations
Katsumasa Fujita, Osaka Univ. (Japan)
Jun Ando, Osaka Univ. (Japan)
Sawako Ishitobi, Osaka Univ. (Japan)
Keisaku Hamada, Osaka Univ. (Japan)
Nicholas I. Smith, Osaka Univ. (Japan)
Yasushi Inouye, Osaka Univ. (Japan)
Satoshi Kawata, Osaka Univ. (Japan)
RIKEN (Japan)


Published in SPIE Proceedings Vol. 7192:
Plasmonics in Biology and Medicine VI
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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