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Proceedings Paper

Temperature impact on thermodynamical parameters of DNA:DNA hybridization kinetics as quantified by Surface Plasmon Resonance Imaging (SPRI) system
Author(s): Jérome Hottin; Julien Moreau; Michael Canva
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Paper Abstract

Surface plasmon resonance imaging optical biochips have shown promising applications in the field of genetic diagnosis due to their ability to record hundred of different biomolecular interactions without any label and in real time. Single nucleotide polymorphisms can be detected using this technique. To improve the accuracy and the reliability of biochips a quantitative study on the impact of temperature on DNA hybridization and secondary structures is shown for various oligonucleotides sequences. In this proceeding we focus on the impact of temperature T on the binding rate kinetic τ, using the model case of DNA:DNA interactions. We show that SPRI can quantify such characteristics as τ(T) and that they follow the Langmuir model variation law.

Paper Details

Date Published: 20 March 2009
PDF: 9 pages
Proc. SPIE 7192, Plasmonics in Biology and Medicine VI, 71920C (20 March 2009); doi: 10.1117/12.808966
Show Author Affiliations
Jérome Hottin, Institut d'Optique (France)
Julien Moreau, Institut d'Optique (France)
Michael Canva, Institut d'Optique (France)


Published in SPIE Proceedings Vol. 7192:
Plasmonics in Biology and Medicine VI
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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