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Proceedings Paper

Polarization microscope by single polarization modulation method: novel instrument for visualizing cytoskeleton
Author(s): In Hee Shin; Sang-Mo Shin; Namhui Ahn; Dug Young Kim
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Paper Abstract

A novel and simplest polarization microscope by single polarization modulation method to visualize cytoskeletal structure in bio-cells have been developed. The polarization microscope visualizes cytoskeletal structure based on birefringence characteristics of cells. In the polarization microscope, a series of intensity images according to single modulation of rotating linear polarizer were obtained to visualize cytoskeletal structure. Phase retardation and principal axis orientation were calculated by analytic data processing from the acquired intensity images. Phase retardation and principal axis orientation of breast cancer cells, MCF 7 and MDA MB 231 were measured and visualized as their birefringence characteristics by the polarization microscope. Phase retardation sensitivity of the polarization microscope system also was measured in this paper.

Paper Details

Date Published: 13 February 2009
PDF: 8 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71821Y (13 February 2009); doi: 10.1117/12.808930
Show Author Affiliations
In Hee Shin, Gwangju Institute of Science and Technology (Korea, Republic of)
Sang-Mo Shin, Gwangju Institute of Science and Technology (Korea, Republic of)
Namhui Ahn, Gwangju Institute of Science and Technology (Korea, Republic of)
Dug Young Kim, Gwangju Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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