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Proceedings Paper

Polarization sensitive optical coherence tomography in equine bone
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Paper Abstract

Optical coherence tomography (OCT) has been used to image equine bone samples. OCT and polarization sensitive OCT (PS-OCT) images of equine bone samples, before and after demineralization, are presented. Using a novel approach, taking a series of images at different angles of illumination, the polar angle and true birefringence of collagen within the tissue is determined, at one site in the sample. The images were taken before and after the bones were passed through a demineralization process. The images show an improvement in depth penetration after demineralization allowing better visualization of the internal structure of the bone and the optical orientation of the collagen. A quantitative measurement of true birefringence has been made of the bone; true birefringence was shown to be 1.9x10-3 before demineralization increasing to 2.7x10-3 after demineralization. However, determined collagen fiber orientation remains the same before and after demineralization. The study of bone is extensive within the field of tissue engineering where an understanding of the internal structures is essential. OCT in bone, and improved depth penetration through demineralization, offers a useful approach to bone analysis.

Paper Details

Date Published: 19 February 2009
PDF: 10 pages
Proc. SPIE 7166, Optics in Bone Biology and Diagnostics, 716608 (19 February 2009); doi: 10.1117/12.808858
Show Author Affiliations
J. W. Jacobs, Kroto Research Institute (United Kingdom)
S. J. Matcher, Kroto Research Institute (United Kingdom)


Published in SPIE Proceedings Vol. 7166:
Optics in Bone Biology and Diagnostics
Andreas Mandelis, Editor(s)

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