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Proceedings Paper

Selective cell culture on UV-transparent polymer by F2 laser modification
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Paper Abstract

A microchip made of UV transparent polymer (CYTOP) that can perform selective cell culture has been fabricated by F2 laser surface modification. The refractive index of CYTOP is almost the same as that of culture medium, which is essential for three dimensional (3D) observation of cells. The F2 laser modification of CYTOP achieves hydrophilicity only on the laser irradiated area with little deterioration of the optical properties and surface smoothness. After the laser modification, HeLa cells were successfully cultured and strongly adhered only on the modified area of CYTOP. The cells patterned on CYTOP were applied for clear 3D observation using an optical microscope in phase contrast mode.

Paper Details

Date Published: 24 February 2009
PDF: 8 pages
Proc. SPIE 7201, Laser Applications in Microelectronic and Optoelectronic Manufacturing VII, 72010X (24 February 2009); doi: 10.1117/12.808815
Show Author Affiliations
Y. Hanada, RIKEN-Advanced Science Institute (Japan)
K. Sugioka, RIKEN-Advanced Science Institute (Japan)
H. Kawano, RIKEN-Brain Science Institute (Japan)
A. Miyawaki, RIKEN-Brain Science Institute (Japan)
K. Midorikawa, RIKEN-Advanced Science Institute (Japan)

Published in SPIE Proceedings Vol. 7201:
Laser Applications in Microelectronic and Optoelectronic Manufacturing VII
Michel Meunier; Andrew S. Holmes; Hiroyuki Niino; Bo Gu, Editor(s)

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