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Proceedings Paper

Toxicity of carbon group quantum dots
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Paper Abstract

Carbon group quantum dots (QDs) such as carbon, silicon and germanium, have potential for biomedical applications such as bio-imaging markers and drug delivery systems and are expected to demonstrate several advantages over conventional fluorescent QDs such as CdSe, especially in biocompatibility. We assessed biocompatibility of newly manufactured silicon QDs (Si-QDs), by means of both MTT assay and LDH assay for HeLa cells in culture and thereby detected the cellular toxicity by administration of high concentration of Si-QD (>1000 μg/mL), while we detected the high toxicity by administration of over 100 μg/mL of CdSe-QDs. As a hypothesis for the cause of the cellular toxicity, we measured oxy-radical generation from the QDs by means of luminol reaction method. We detected generation of oxy-radicals from the Si-QDs and those were decreased by radical scavenger such as superoxide dismutase (SOD) and N-acetyl cysteine (NAC). We concluded that the Si-QD application to cultured cells in high concentration led cell membrane damage by oxy-radicals and combination usage with radical scavenger is one of the answers.

Paper Details

Date Published: 3 March 2009
PDF: 8 pages
Proc. SPIE 7189, Colloidal Quantum Dots for Biomedical Applications IV, 71891A (3 March 2009); doi: 10.1117/12.808805
Show Author Affiliations
Sanshiro Hanada, International Medical Ctr. of Japan (Japan)
Kouki Fujioka, International Medical Ctr. of Japan (Japan)
Jikei Univ. School of Medicine (Japan)
Akiyoshi Hoshino, International Medical Ctr. of Japan (Japan)
Noriyoshi Manabe, International Medical Ctr. of Japan (Japan)
Kenji Hirakuri, Tokyo Denki Univ. (Japan)
Kenji Yamamoto, International Medical Ctr. of Japan (Japan)

Published in SPIE Proceedings Vol. 7189:
Colloidal Quantum Dots for Biomedical Applications IV
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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