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Proceedings Paper

Spectral phase shaping for high resolution CARS spectroscopy around 3000 cm-1
Author(s): A. C. W. van Rhijn; S. Postma; J. P. Korterik; J. L. Herek; H. L. Offerhaus
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Paper Abstract

By spectral phase shaping of both the pump and probe pulses in coherent anti-Stokes Raman scattering (CARS) spectroscopy we demonstrate the extraction of the frequencies of vibrational lines using an unamplified oscillator. Furthermore we demonstrate chemically selective broadband CARS microscopy on a mixed sample of 4 μm diameter polystyrene (PS) and poly(methyl methacrylate) (PMMA) beads. The CARS signal from either the PS or the PMMA beads is shown to be enhanced or suppressed, depending on the phase profile applied to the broadband spectrum. Using a combination of negative and positive (sloped) π-phase steps in the pump and probe spectrum the purely non-resonant background signal is removed.

Paper Details

Date Published: 13 February 2009
PDF: 8 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830X (13 February 2009); doi: 10.1117/12.808792
Show Author Affiliations
A. C. W. van Rhijn, Univ. Twente (Netherlands)
S. Postma, Univ. Twente (Netherlands)
J. P. Korterik, Univ. Twente (Netherlands)
J. L. Herek, Univ. Twente (Netherlands)
H. L. Offerhaus, Univ. Twente (Netherlands)


Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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