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Proceedings Paper

Investigation on fibrous collagen modifications during corneal laser welding by second harmonic generation microscopy
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Paper Abstract

The structural modifications of the collagen lattice induced in corneal stroma by laser welding were investigated with second-harmonic-generation (SHG) imaging. Corneal laser welding lies in the staining of the wound with a saturated solution of ICG followed by irradiation with a near-infrared-laser operated in continuous (CWLW) or pulsed (PWL) mode. After CWLW the lamellar arrangement was lost although a densely packing of collagen bundles increasingly disordered remained still clearly visible pointing out the lack of complete collagen denaturation. PLW produced welding spots which were characterized by a severe loss of SHG signal suggesting the occurrence of a complete collagen denaturation.

Paper Details

Date Published: 18 February 2009
PDF: 4 pages
Proc. SPIE 7163, Ophthalmic Technologies XIX, 71630Y (18 February 2009); doi: 10.1117/12.808739
Show Author Affiliations
Paolo Matteini, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)
Fulvio Ratto, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)
Francesca Rossi, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)
Riccardo Cicchi, Univ. degli Studi di Firenze (Italy)
Chiara Stringari, Univ. degli Studi di Firenze (Italy)
Dimitrios Kapsokalyvas, Univ. degli Studi di Firenze (Italy)
Francesco S. Pavone, Univ. degli Studi di Firenze (Italy)
Roberto Pini, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)


Published in SPIE Proceedings Vol. 7163:
Ophthalmic Technologies XIX
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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