Share Email Print
cover

Proceedings Paper

Relative concentration of fluorophores in a scattering medium using fluorescence lifetime contrast
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In vivo optical molecular imaging of fluorescent probes predominantly employs continuous wave techniques to measure fluorescence intensity. Alternatively, time domain techniques permit measurement of fluorescence lifetime in addition to fluorescence intensity. Fluorescence lifetime allows discrimination of fluorescent probes with contrasting lifetime or inference of a probe's environment due to lifetime sensitivity. Here, we present the use of fluorescence lifetime contrast to evaluate the relative concentrations of a mixture of fluorophores in a scattering medium. This approach offers the potential to perform dual-probe in vivo optical molecular imaging at a single wavelength employing lifetime contrast rather than via spectral intensity contrast.

Paper Details

Date Published: 21 February 2009
PDF: 6 pages
Proc. SPIE 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, 71900S (21 February 2009); doi: 10.1117/12.808609
Show Author Affiliations
David J. Hall, Univ. of California, San Diego (United States)
Sung-Ho Han, Univ. of California, San Diego (United States)


Published in SPIE Proceedings Vol. 7190:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

© SPIE. Terms of Use
Back to Top