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Proceedings Paper

Multivalent display of DNA conjugates on semiconductor quantum dots utilizing a novel conjugation method
Author(s): Duane E. Prasuhn; Juan B. Blanco-Canosa; Gary J. Vora; Hedi M. Mattoussi; Philip E. Dawson; Igor L. Medintz
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Paper Abstract

One of the most prominent research areas in nanotechnology is the development of nanoparticle systems for biomedical applications. This is founded upon the expectation that such species could ultimately be imbued with multiple simultaneous functions, such as the presentation of a therapeutic payload or diagnostic sensor for in vivo trafficking to desired cell types. In recent years, semiconductor quantum dots (QDs) have been actively explored as novel display systems, because of their unique photophysical properties. Using an aniline-mediated hydrazone coupling, a polyhisitidine-appended peptide was derivatized with a DNA strand and successfully self-assembled to QDs, yielding nanoparticles displaying up to approximately 15 peptide/DNA conjugates. This ligation method is a viable chemistry for displaying biomolecules, because of the orthogonality of the ketone and hydrazine moieties to most biological functionality and the reaction can be performed under mild conditions in aqueous media. The modified QDs were further characterized by gel electrophoresis, and microarray studies; showing the self-assembly was successful and the DNA strands were still available for hybridization with a complement sequence.

Paper Details

Date Published: 3 March 2009
PDF: 7 pages
Proc. SPIE 7189, Colloidal Quantum Dots for Biomedical Applications IV, 71890I (3 March 2009); doi: 10.1117/12.808541
Show Author Affiliations
Duane E. Prasuhn, U.S. Naval Research Lab. (United States)
Juan B. Blanco-Canosa, U.S. Naval Research Lab. (United States)
Gary J. Vora, U.S. Naval Research Lab. (United States)
Hedi M. Mattoussi, The Scripps Research Inst. (United States)
Philip E. Dawson, U.S. Naval Research Lab. (United States)
Igor L. Medintz, U.S. Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 7189:
Colloidal Quantum Dots for Biomedical Applications IV
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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