Share Email Print
cover

Proceedings Paper

Peptide linkers for the assembly of semiconductor quantum dot bioconjugates
Author(s): Kelly Boeneman; Bing C. Mei; Jeffrey R. Deschamps; James B. Delehanty; Hedi Mattoussi; Igor Medintz
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The use of semiconductor luminescent quantum dots for the labeling of biomolecules is rapidly expanding, however it still requires facile methods to attach functional globular proteins to biologically optimized quantum dots. Here we discuss the development of controlled variable length peptidyl linkers to attach biomolecules to poly(ethylene) glycol (PEG) coated quantum dots for both in vitro and in vivo applications. The peptides chosen, β-sheets and alpha helices are appended to polyhistidine sequences and this allows for control of the ratio of peptide bioconjugated to QD and the distance from QD to the biomolecule. Recombinant DNA engineering, bacterial peptide expression and Ni-NTA purification of histidine labeled peptides are utilized to create the linkers. Peptide length is confirmed by in vitro fluorescent resonance energy transfer (FRET).

Paper Details

Date Published: 3 March 2009
PDF: 9 pages
Proc. SPIE 7189, Colloidal Quantum Dots for Biomedical Applications IV, 71890G (3 March 2009); doi: 10.1117/12.808540
Show Author Affiliations
Kelly Boeneman, U.S. Naval Research Lab. (United States)
Bing C. Mei, U.S. Naval Research Lab. (United States)
Jeffrey R. Deschamps, U.S. Naval Research Lab. (United States)
James B. Delehanty, U.S. Naval Research Lab. (United States)
Hedi Mattoussi, U.S. Naval Research Lab. (United States)
Igor Medintz, U.S. Naval Research Lab. (United States)


Published in SPIE Proceedings Vol. 7189:
Colloidal Quantum Dots for Biomedical Applications IV
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

© SPIE. Terms of Use
Back to Top