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Proceedings Paper

In vivo photoacoustic monitoring of photosensitizer in skin: application to dosimetry for antibacterial photodynamic treatment
Author(s): Akihiro Hirao; Shunichi Sato; Daizoh Saitoh; Nariyoshi Shinomiya; Hiroshi Ashida; Minoru Obara
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Paper Abstract

To obtain efficient antibacterial photodynamic effect in traumatic injuries such as burns, depth-resolved dosimetry of photosensitizer is required. In this study, we performed dual-wavelength photoacoustic (PA) measurement for rat burned skins injected with a photosensitizer. As a photosensitizer, methylene blue (MB) or porfimer sodium was injected into the subcutaneous tissue in rats with deep dermal burn. The wound was irradiated with red (665 nm or 630 nm) pulsed light to excite photosensitizers and green (532 nm) pulsed light to excite blood in the tissue; the latter signal was used to eliminate blood-associated component involved in the former signal. Acoustic attenuation was also compensated from the photosensitizer-associated PA signals. These signal processing was effective to obtain high-contrast image of a photosensitizer in the tissue. Behaviors of MB and porfimer sodium in the tissue were compared.

Paper Details

Date Published: 12 February 2009
PDF: 7 pages
Proc. SPIE 7177, Photons Plus Ultrasound: Imaging and Sensing 2009, 71772C (12 February 2009); doi: 10.1117/12.808480
Show Author Affiliations
Akihiro Hirao, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Daizoh Saitoh, National Defense Medical College (Japan)
Nariyoshi Shinomiya, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College (Japan)
Minoru Obara, Keio Univ. (Japan)


Published in SPIE Proceedings Vol. 7177:
Photons Plus Ultrasound: Imaging and Sensing 2009
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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