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Proceedings Paper

Two-photon in vivo flow cytometry using a fiber probe
Author(s): Yu-Chung Chang; Jing Yong Ye; Thommey P. Thomas; Zhengyi Cao; Alina Kotlyar; Eric R. Tkaczyk; James R. Baker Jr.; Theodore B. Norris
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Paper Abstract

We have demonstrated the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in vitro and in vivo. We conducted two-channel detection to measure fluorescence at two distinct wavelengths simultaneously. Because the scattering and absorption problems from whole blood were circumvented by the fiber probe, the detected signal strength from the cells were found to be similar in PBS and in whole blood. We achieved the same detection efficiency of the membrane-binding lipophilic dye DiD labeled cells in PBS and in whole blood. High detection efficiency of green fluorescent protein (GFP)-expressing cells in whole blood was demonstrated. DiD-labeled untransfected and GFP-transfected cells were injected into live mice and the circulation dynamics of the externally injected cells were monitored. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed in whole blood.

Paper Details

Date Published: 20 February 2009
PDF: 10 pages
Proc. SPIE 7173, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX, 71730I (20 February 2009); doi: 10.1117/12.808436
Show Author Affiliations
Yu-Chung Chang, Univ. of Michigan (United States)
Jing Yong Ye, Univ. of Michigan (United States)
Thommey P. Thomas, Univ. of Michigan (United States)
Zhengyi Cao, Univ. of Michigan (United States)
Alina Kotlyar, Univ. of Michigan (United States)
Eric R. Tkaczyk, Univ. of Michigan (United States)
James R. Baker Jr., Univ. of Michigan (United States)
Theodore B. Norris, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 7173:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX
Israel Gannot, Editor(s)

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