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Proceedings Paper

Second harmonic generating (SHG) nanoprobes: a new tool for biomedical imaging
Author(s): Periklis Pantazis; Ye Pu; Demetri Psaltis; Scott Fraser
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Paper Abstract

Fluorescence microscopy has profoundly changed how cell and molecular biology is studied in almost every aspect. However, the need of characterizing biological targets is largely unmet due to deficiencies associated with the use of fluorescent agents. Dye bleaching, dye signal saturation, blinking, and tissue autofluorescence can severely limit the signal-to-noise ratio (SNR). Given the photophysical properties are fundamentally different to the fluorescent agents currently used in biomedical research, second harmonic generating (SHG) nanoprobes can be suitable for biomedical imaging and can eliminate most of the drawbacks encountered in classical fluorescence systems.

Paper Details

Date Published: 13 February 2009
PDF: 5 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831P (13 February 2009); doi: 10.1117/12.808434
Show Author Affiliations
Periklis Pantazis, California Institute of Technology (United States)
Ye Pu, California Institute of Technology (United States)
Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Demetri Psaltis, California Institute of Technology (United States)
Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Scott Fraser, California Institute of Technology (United States)


Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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