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Proceedings Paper

Ultrafast multiphoton microscopy with high-order spectral phase distortion compensation
Author(s): Yair Andegeko; Dmitry Pestov; Vadim V. Lozovoy; Marcos Dantus
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Paper Abstract

High-order dispersion of ultrashort laser pulses (with ~100 nm bandwidth) is shown to account for significant reduction of two-photon excitation fluorescence and second harmonic generation signal produced at the focal plane of a laser-scanning two-photon microscope. The second- and third-order corrections recover 20-40% of the signal intensity expected for a transform-limited laser pulse, while the rest depends on the proper compensation of higher-order terms. It can be accomplished through the use of a pulse shaper by measuring and correcting all nonlinear spectral phase distortions.

Paper Details

Date Published: 13 February 2009
PDF: 6 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830W (13 February 2009); doi: 10.1117/12.808429
Show Author Affiliations
Yair Andegeko, Michigan State Univ. (United States)
Dmitry Pestov, Michigan State Univ. (United States)
Vadim V. Lozovoy, Michigan State Univ. (United States)
Marcos Dantus, Michigan State Univ. (United States)


Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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