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A new light source for multimodal multiphoton microscopy including CARSFormat | Member Price | Non-Member Price |
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Paper Abstract
We will present a new flexible laser source for multimodal Multiphoton excitation microscopy including CARS. It
consists of a tuneable femtosecond-Ti:Sapphire laser and an optical parametric oscillator (OPO). The new OPO-design
allows for high flexibility in pump- and output wavelengths giving rise to for instance image EGFP with the Ti:Sapphire
and tdRFP with the OPO simultaneously. This is presented on living mouse brain tissue.
The minimum energy difference between Ti:Sapphire and
OPO-wavelengths achievable is 2500cm-1. Thus CARS
imaging of lipids is possible. Due to synchronous pumping of the OPO the pump- and OPO pulses are intrinsically
locked in time to each other thus they can be brought to perfect overlap of pump and stokes pulses. Uncaging
multiphoton microscopy is also possible with this system due to the low minimum OPO pump wavelength of 730nm.
Paper Details
Date Published: 13 February 2009
PDF: 7 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718314 (13 February 2009); doi: 10.1117/12.808376
Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)
PDF: 7 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718314 (13 February 2009); doi: 10.1117/12.808376
Show Author Affiliations
Ingo Rimke, APE GmbH (Germany)
Volker Siffrin, Univ. Berlin (Germany)
Raluca Niesner, Univ. Berlin (Germany)
Tina Leuenberger, Univ. Berlin (Germany)
Volker Siffrin, Univ. Berlin (Germany)
Raluca Niesner, Univ. Berlin (Germany)
Tina Leuenberger, Univ. Berlin (Germany)
Frauke Zipp, Univ. Berlin (Germany)
Edlef Büttner, APE GmbH (Germany)
Gero Stibenz, APE GmbH (Germany)
Edlef Büttner, APE GmbH (Germany)
Gero Stibenz, APE GmbH (Germany)
Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)
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