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Proceedings Paper

Imaging EGFR distribution using surface-enhanced Raman spectroscopy
Author(s): L. Lucas; X. K. Chen; A. Smith; M. Korbelik; H. Zeng; P. W. K. Lee; K. C. Hewitt
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Paper Abstract

The purpose of this study is to explore the feasibility of using Surface Enhanced Raman Spectroscopy (SERS) to image the distribution of Epidermal Growth Factor Receptor (EGFR) in cells. To accomplish this task, 30 nm gold nanoparticles (AuNPs) tagged with antibodies to EGFR (1012 per ml) are incubated with cells (106 per ml) of the A431 human epidermoid carcinoma cell line and normal human bronchial epithelial (NHBE) cells. Using the 632.8 nm excitation line of a He-Ne laser, Raman spectroscopy measurements are performed using a point mapping scheme. SERS signals are observed with an overall enhancement of 4-7 orders of magnitude. Raman intensity maps of the 1480 and 1583 cm-1 peaks correlate well with the expected distribution of AuNPs and EGFR. Normal cells show little to no enhancement. The results therefore present a simple yet effective means to image EGFR over-expression.

Paper Details

Date Published: 18 February 2009
PDF: 8 pages
Proc. SPIE 7192, Plasmonics in Biology and Medicine VI, 719206 (18 February 2009); doi: 10.1117/12.808337
Show Author Affiliations
L. Lucas, Dalhousie Univ. (Canada)
X. K. Chen, Simon Fraser Univ. (Canada)
A. Smith, Dalhousie Univ. (Canada)
M. Korbelik, British Columbia Cancer Research Ctr. (Canada)
H. Zeng, British Columbia Cancer Research Ctr. (Canada)
P. W. K. Lee, Dalhousie Univ. (Canada)
K. C. Hewitt, Dalhousie Univ. (Canada)

Published in SPIE Proceedings Vol. 7192:
Plasmonics in Biology and Medicine VI
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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