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Proceedings Paper

Probing the mechanical properties of Drosophila embryo ephitelial cells in vivo by laser nanodissection
Author(s): Matteo Rauzi; Eric P. Mottay; Thomas Lecuit; Pierre-François Lenne
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Paper Abstract

We used laser nanodissection to study the nature, magnitude and distribution of forces at cell-cell contacts during tissue morphogenesis in live Drosophila embryos. We designed two set-ups coupling a near infrared femtosecond laser to either an inverted fluorescence microscope or a spinning-disk confocal microscope. We show that the developed tools are able to locally disrupt the acto-myosin network while preserving the integrity of the membranes. With these systems, we could explore the redistribution of cortical elements and relaxation of cell-cell contacts after local ablation.

Paper Details

Date Published: 13 February 2009
PDF: 6 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71820Y (13 February 2009); doi: 10.1117/12.808171
Show Author Affiliations
Matteo Rauzi, Institut de Biologie du Développement de Marseille Luminy, CNRS (France)
Institut Fresnel, CNRS, Univ. Aix Marseille (France)
Eric P. Mottay, Amplitude Systemes (France)
Thomas Lecuit, Institut de Biologie du Développement de Marseille Luminy, CNRS (France)
Pierre-François Lenne, Institut de Biologie du Développement de Marseille Luminy, CNRS (France)
Institut Fresnel, CNRS, Univ. Aix Marseille (France)


Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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