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Proceedings Paper

In vivo optical imaging to visualize photodynamic therapy-induced immune responses
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Paper Abstract

Motivated by recent successes in growing intradermal tumors in the ears of mice and establishing the feasibility of in vivo confocal imaging of anatomic vessels in these tumors using fluorophore-conjugated antibodies to CD31, we are exploring a number of applications of optical fluorescence imaging in superficial murine tumor models in vivo. Immune responses induced by photodynamic therapy (PDT) are dynamic processes that occur in a spatially and temporally specific manner. To visualize these processes noninvasively, we have made progress in developing optical molecular imaging strategies that take advantage of intradermal injection of fluorophore-conjugated-antibodies against surface antigens on immune cells. This enables confocal imaging of the fluorescently labeled host cells to depths of at least 100 microns, and using this technique we have achieved in vivo imaging of granulocyte (GR-1)- and major histocompatibility complex class II (MHC-II)-positive cell trafficking in tumors in response to PDT. The latter include macrophages and dendritic cells. Data from tumors that were subjected to PDT with the photosensitizer, HPPH, reveals a significantly enhanced level of GR-1+ cell infiltration compared to untreated control tumor. The temporal kinetics of GR-1+ and MHC-II+ cells at different time intervals post-PDT are being examined. The ability to image host responses in vivo without excising or perturbing the tissue has opened up opportunities to explore means of optimizing them to therapeutic advantage.

Paper Details

Date Published: 25 February 2009
PDF: 8 pages
Proc. SPIE 7178, Biophotonics and Immune Responses IV, 71780F (25 February 2009); doi: 10.1117/12.808117
Show Author Affiliations
Soumya Mitra, Univ. of Rochester (United States)
Thomas H. Foster, Univ. of Rochester (United States)

Published in SPIE Proceedings Vol. 7178:
Biophotonics and Immune Responses IV
Wei R. Chen, Editor(s)

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