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Proceedings Paper

Deep high-resolution fluorescence microscopy of full organs: the benefit of ultraminiature confocal miniprobes
Author(s): France Schwarz; Arnaud Le Nevez; Magalie Genet; Anne Osdoit; François Lacombe
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Paper Abstract

Background: Confocal Laser Endomicroscopy (CLE) based on ultraminiature miniprobes (Cellvizio®, Mauna Kea Technologies, Paris, France) is able to image the inner microstructure of retroperitoneal full organs punctured during EUS-FNA procedures, such as pancreas, liver or lymph nodes. Therefore, pCLE can provide an easy-to-use and precise adjunct tool to ultrasonographic interventions in order to target suspicious areas for biopsies in EUS-FNA. Material and Methods: Probe-based CLE (pCLE) was performed on ex-vivo surgically resected specimens after topical application of fluorophores in standard 19G and 22G needles. Two prototype miniprobes ("S-probe" 300 microns diameter, field of view 400*280 microns, and "S-probe" 650 microns diameter, field of view 500*600 microns) were then inserted into the needles and enabled visualization of the inner microstructures of uterus, lung, kidney, stomach and esophagus, in both healthy and cancerous conditions. Then, pCLE was performed in-vivo on four pigs during three NOTES and one EUS-FNA procedures after intravenous injection of 2-7mL fluorescein 1-10% using the prototype "S-probe" 350 microns diameter inserted in 19G FNA needles. Liver, pancreas and spleen were imaged. Results: During the ex-vivo experiments, pCLE made it possible to distinguish microstructures, such as alveoli and macrophages in the lungs. During the in-vivo experiments, Cellvizio® video sequences showed hepatic lobules and the portal vein in the liver, and red and white pulp in the spleen. Conclusion: pCLE provides in vivo cellular information about full organs. It has the potential to help target biopsies during EUSFNA, which suffers from a high rate of false negatives, thus increasing its sensitivity.

Paper Details

Date Published: 2 March 2009
PDF: 11 pages
Proc. SPIE 7172, Endoscopic Microscopy IV, 71720G (2 March 2009); doi: 10.1117/12.808108
Show Author Affiliations
France Schwarz, Mauna Kea Technologies (France)
Arnaud Le Nevez, Mauna Kea Technologies (France)
Magalie Genet, Mauna Kea Technologies (France)
Anne Osdoit, Mauna Kea Technologies (France)
François Lacombe, Mauna Kea Technologies (France)

Published in SPIE Proceedings Vol. 7172:
Endoscopic Microscopy IV
Guillermo J. Tearney; Thomas D. Wang, Editor(s)

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