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Proceedings Paper

Ultra-compact (palm-top size) low-cost maintenance-free (>3000 h) diode-pumped femtosecond (160 fs) solid state laser source for multiphoton microscopy
Author(s): S. Yamazoe; M. Katou; T. Kasamatsu
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Paper Abstract

We propose and demonstrate a novel practical femtosecond laser source, which is to our knowledge, the smallest size and potentially low cost. The innovation is the simple linear cavity design utilizing soliton mode-locking induced by precise group velocity dispersion control. Average output power of 680 mW and pulse width of 162 fs were obtained at around 1045 nm from a diode-pumped Yb3+:KY(WO4)2 laser. The pulse repetition rate was 2.8 GHz, leading to a pulse peak power of 1.5 kW, which is sufficient for biomedical imaging. The laser module including the laser diode pump system has a footprint of 8×4 cm2. Under automatic current control condition, stable operation of 3000-hour was observed with an average power fluctuation of less than ±10 %. Furthermore, under automatic power control condition, stable operation of 2000-hour was observed with an average power fluctuation of less than ±1 %. Using this laser module, we successfully obtained clear two-photon fluorescence images of muntjac skin fibroblast cells stained with a combination of fluorescent stains (Alexa Fluor 488 phalloidin and Alexa Flour 555 goat anti-mouse lgG).

Paper Details

Date Published: 25 February 2009
PDF: 10 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718334 (25 February 2009); doi: 10.1117/12.808082
Show Author Affiliations
S. Yamazoe, Fujifilm Corp. (Japan)
M. Katou, Fujifilm Corp. (Japan)
T. Kasamatsu, Fujifilm Corp. (Japan)


Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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