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Proceedings Paper

Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature
Author(s): Myunghwan Choi; Taesu Chung; Kyungsun Choi; Chulhee Choi
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Paper Abstract

Angiogenesis is essential for tumor growth and a promising target for cancer therapy. Blood vessel monitoring is an indispensable tool for evaluation and development of anti-angiogenic drugs. Here, we report a new noninvasive in vivo imaging tool, named dynamic fluorescence imaging (DyFI), for the simultaneous measurement of multiple vascular parameters, such as vascular density, perfusion rate, and permeability, using spatiotemporal profiles of indocyanine green. Using DyFI in a tumor xenograft model, we quantitatively measured multiple vascular parameters in tumors and normal tissues with high spatial resolution. The multimodality of this method allowed us to find a negative spatial correlation between perfusion and permeability. Moreover, DyFI was effective for revealing the early effects of an anti-angiogenic drug; these findings were validated using two-photon microscopy. We suggest that DyFI could be a useful tool for the preclinical development of anti-angiogenic drugs.

Paper Details

Date Published: 12 February 2009
PDF: 5 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71821O (12 February 2009); doi: 10.1117/12.808081
Show Author Affiliations
Myunghwan Choi, Korea Advanced Institute of Science and Technology (Korea, Republic of)
Taesu Chung, Korea Advanced Institute of Science and Technology (Korea, Republic of)
Kyungsun Choi, Korea Advanced Institute of Science and Technology (Korea, Republic of)
Chulhee Choi, Korea Advanced Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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