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Proceedings Paper

A noncytotoxic DsRed variant for whole-cell labeling
Author(s): Rita L. Strack; Daniel E. Strongin; Dibyendu Bhattacharyya; Wen Tao; Allison Berman; Hal E. Broxmeyer; Robert J. Keenan; Benjamin S. Glick
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Paper Abstract

Fluorescent proteins (FPs) are extremely useful tools for whole-cell, tissue, and animal labeling. For these purposes, FPs may be monomeric or oligomeric, but should meet the criteria of being tolerated at high expression levels in cells and having desirable photophysical properties. Our goal was to create a variant of DsRed-Express that maintains the brightness, fast-maturation, and photostability of this protein, while exhibiting decreased cytotoxicity. For this purpose, we mutated the surface of DsRed-Express to decrease aggregation and created DsRed-Express2. DsRed-Express2 retains the favorable photophysical properties of DsRed-Express while showing dramatically reduced cytotoxicity and higher expression in bacterial and mammalian systems. Further, it was shown that DsRed-Express2 outperforms other red FPs as a label for bacterial and mammalian cells.

Paper Details

Date Published: 17 February 2009
PDF: 12 pages
Proc. SPIE 7191, Fluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV, 719109 (17 February 2009); doi: 10.1117/12.808046
Show Author Affiliations
Rita L. Strack, The Univ. of Chicago (United States)
Daniel E. Strongin, The Univ. of Chicago (United States)
Dibyendu Bhattacharyya, The Univ. of Chicago (United States)
Wen Tao, Indiana Univ. School of Medicine (United States)
Allison Berman, The Univ. of Chicago (United States)
Hal E. Broxmeyer, Indiana Univ. School of Medicine (United States)
Robert J. Keenan, The Univ. of Chicago (United States)
Benjamin S. Glick, The Univ. of Chicago (United States)


Published in SPIE Proceedings Vol. 7191:
Fluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV
Alexander P. Savitsky; Yingxiao Wang, Editor(s)

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