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Proceedings Paper

Quantitative phase-contrast digital holographic microscopy for cell dynamic evaluation
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Paper Abstract

The laser microbeam uses lasers to alter and/or to ablate intracellular organelles and cellular and tissue samples, and, today, has become an important tool for cell biologists to study the molecular mechanism of complex biological systems by removing individual cells or sub-cellular organelles. However, absolute quantitation of the localized alteration/damage to transparent phase objects, such as the cell membrane or chromosomes, was not possible using conventional phase-contrast or differential interference contrast microscopy. We report the development of phase-contrast digital holographic microscopy for quantitative evaluation of cell dynamic changes in real time during laser microsurgery. Quantitative phase images are recorded during the process of laser microsurgery and thus, the dynamic change in phase can be continuously evaluated. Out-of-focus organelles are re-focused by numerical reconstruction algorithms.

Paper Details

Date Published: 3 February 2009
PDF: 6 pages
Proc. SPIE 7233, Practical Holography XXIII: Materials and Applications, 72330C (3 February 2009); doi: 10.1117/12.808038
Show Author Affiliations
Lingfeng Yu, Univ. of California, Irvine (United States)
Samarendra Mohanty, Univ. of California, Irvine (United States)
Michael W. Berns, Univ. of California, Irvine (United States)
Zhongping Chen, Univ. of California, Irvine (United States)


Published in SPIE Proceedings Vol. 7233:
Practical Holography XXIII: Materials and Applications
Hans I. Bjelkhagen; Raymond K. Kostuk, Editor(s)

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