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Proceedings Paper

Cellular imagery with total internal reflection holographic microscopy
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Paper Abstract

Evanescent wave phase profiling is incorporated with digital holography into a new surface imaging technique termed total internal reflection holographic microscopy (TIRHM). Quantitative images of tissue structures and profiles of cellular membranes are presented to demonstrate the method's performance capabilities. Applications of this technique include measurements of cellular membranes and their transport processes without the addition of fluorophores. The angular spectrum method to compensate for tilt anamorphism due to the inclined TIR plane is also discussed.

Paper Details

Date Published: 13 February 2009
PDF: 8 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71820A (13 February 2009); doi: 10.1117/12.808029
Show Author Affiliations
William M. Ash, Univ. of South Florida (United States)
Myung K. Kim, Univ. of South Florida (United States)


Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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