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Proceedings Paper

The role of alkanethiol spacers in a metal surface-based label-free DNA detection system
Author(s): Hsin-I Peng; Zachary M. Nuffer; Benjamin L. Miller
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Paper Abstract

We have been developing an array-based DNA biosensor, in which DNA oligonucleotides are readily immobilized onto Au surfaces via terminal thiol groups to serve as probes for the detection of oligonucleotides in solution. One common strategy to minimize steric effects and non-specific oligonucleotide-surface interactions derived from a high surface probe density employs thiol molecules as spacers. In this study, we tested nine different alkanethiol molecules with the goal of identifying a relationship between the chemical structure of each molecule and its performance as a spacer molecule. Among the nine different spacers, 4-mercapto-1-butanol and 1-decanethiol were found to yield the optimal performance as spacers, while spacers with a bulky tert-butyl group or a ring structure (2-methyl-2-propanethiol, cyclohexyl mercaptan and thiophenol) yielded poorer performance. Spacers with a short, branched carbon chain (1- mercapto-2-propanol and 3-mercapto-1,2-propanediol) provided an intermediate performance. These results suggest that DNA sensor response is highly sensitive to the chemical structure of the alkanethiol molecules that are employed as spacer molecules.

Paper Details

Date Published: 19 February 2009
PDF: 8 pages
Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 71670T (19 February 2009); doi: 10.1117/12.808023
Show Author Affiliations
Hsin-I Peng, Univ. of Rochester (United States)
Zachary M. Nuffer, Univ. of Rochester (United States)
Benjamin L. Miller, Univ. of Rochester (United States)

Published in SPIE Proceedings Vol. 7167:
Frontiers in Pathogen Detection: From Nanosensors to Systems
Philippe M. Fauchet, Editor(s)

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