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Proceedings Paper

Analysis of NAD(P)H fluorescence components in cardiac myocytes from human biopsies: a new tool to improve diagnostics of rejection of transplanted patients
Author(s): Y. Cheng; A. Mateasik; N. Poirier; J. Miró; N. Dahdah; D. Chorvat; A. Chorvatova
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Paper Abstract

Tissue autofluorescence is one of the most versatile non-invasive tools for mapping the metabolic state in living tissues. Increasing interest in the imaging and diagnosis of living cells and tissues, based on their intrinsic fluorescence rather than fluorescence labeling, is closely connected to the latest developments in high-performance spectroscopic and microscopic techniques. We investigate metabolic state of cardiac cells isolated from one additional human biopsy from transplanted pediatric patients presenting either no rejection (R0) or mild rejection (R1). Two different approaches for isolation of human cardiac myocytes are also compared. Spectrally-resolved fluorescence lifetime detection of NAD(P)H fluorescence (excitation by pulsed 375 nm picosecond laser) is tested as a promising new tool for quantitative analysis of intrinsic cellular autofluorescence signals in living cardiomyocytes. This work opens new horizons in the evaluation of cardiac transplant rejection using latest fluorescence imaging approaches.

Paper Details

Date Published: 25 February 2009
PDF: 8 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830K (25 February 2009); doi: 10.1117/12.808001
Show Author Affiliations
Y. Cheng, CHU Sainte-Justine (Canada)
A. Mateasik, International Laser Ctr. (Slovakia)
N. Poirier, CHU Sainte-Justine (Canada)
Univ. of Montreal (Canada)
J. Miró, CHU Sainte-Justine (Canada)
Univ. of Montreal (Canada)
N. Dahdah, CHU Sainte-Justine (Canada)
Univ. of Montreal (Canada)
D. Chorvat, International Laser Ctr. (Slovakia)
A. Chorvatova, CHU Sainte-Justine (Canada)
Univ. of Montreal (Canada)

Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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