
Proceedings Paper
Neurobiological use of a micro-optrode using UV excitation light and signal-to-noise ratio optimizationFormat | Member Price | Non-Member Price |
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Paper Abstract
μMany fluorescent tools have been developed through the past decades in order to better understand the physiology at a
cellular level. They are generally used for microscopy or endoscopy, in vivo and in vitro, but they are also usable with
fluorescent sensors such as fiber optic sensors. Among these tools, fluorescent ion indicators have been widely used to
understand ionic dynamics into living cells. Indicators are mainly designed for Ca2+, although K+ is also an important
target for its role in maintaining cellular membrane potential and regulating many other electrophysiological phenomena.
Here we propose a technique to improve the use of a miniature fiber optic sensor to sense potassium dynamics in vivo.
Due to the lack of commercially available potassium indicators, we are using a UV excitable indicator (PBFI). The UV
excitation light induces unwanted fluorescence and/or luminescence from the optical components including the sensing
fiber, leading to a poor signal-to-noise ratio. Our technique uses a UV diode pumped Q-switched source from
CrystaLaser, a time gated acquisition system and a delay fiber that improve the signal-to-noise ratio and allow reducing
the sensor size and the light intensity provided to the tissue, thus diminishing UV induced photodamage. Using this
technique we have been able to record intracellular potassium fluctuations in vivo with fiber optic sensors of 10μm
diameter.
Paper Details
Date Published: 23 February 2009
PDF: 9 pages
Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 71613H (23 February 2009); doi: 10.1117/12.807991
Published in SPIE Proceedings Vol. 7161:
Photonic Therapeutics and Diagnostics V
Henry Hirschberg; Brian Jet-Fei Wong; Kenton W. Gregory; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Guillermo J. Tearney; Justus F. R. Ilgner; Steen J. Madsen; Laura Marcu; Haishan Zeng, Editor(s)
PDF: 9 pages
Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 71613H (23 February 2009); doi: 10.1117/12.807991
Show Author Affiliations
Florin Amzica, Univ. Laval (Canada)
Univ. de Montréal (Canada)
Réal Vallée, Univ. Laval (Canada)
Univ. de Montréal (Canada)
Réal Vallée, Univ. Laval (Canada)
Published in SPIE Proceedings Vol. 7161:
Photonic Therapeutics and Diagnostics V
Henry Hirschberg; Brian Jet-Fei Wong; Kenton W. Gregory; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Guillermo J. Tearney; Justus F. R. Ilgner; Steen J. Madsen; Laura Marcu; Haishan Zeng, Editor(s)
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