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Proceedings Paper

Action potential detection by non-linear microscopy
Author(s): Leonardo Sacconi; Jacopo Lotti; Rodney P. O’Connor; Jonathan Mapelli; Daniela Gandolfi; Egidio D'Angelo; Francesco S. Pavone
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Paper Abstract

In this work, we combined the advantages of second-harmonic generation (SHG) with a random access (RA) excitation scheme to realize a new microscope (RA-SHG) capable of optically recording fast membrane potential events occurring in a wide-field configuration. The RA-SHG microscope in combination with a bulk staining method with FM4-64 was used to simultaneously record electrical activity from clusters of Purkinje cells (PCs) in acute cerebellar slices. Spontaneous electrical activity was also monitored simultaneously in pairs of neurons, where APs were recorded in a single trial without averaging. These results show the strength of this technique to describe the temporal dynamics of neuronal assemblies.

Paper Details

Date Published: 23 February 2009
PDF: 11 pages
Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 71613F (23 February 2009); doi: 10.1117/12.807966
Show Author Affiliations
Leonardo Sacconi, European Lab. for Non-linear Spectroscopy, Univ. of Florence (Italy)
Univ. of Florence (Italy)
Jacopo Lotti, European Lab. for Non-linear Spectroscopy, Univ. of Florence (Italy)
Rodney P. O’Connor, European Lab. for Non-linear Spectroscopy, Univ. of Florence (Italy)
Jonathan Mapelli, Univ. of Pavia (Italy)
Daniela Gandolfi, Univ. of Pavia (Italy)
Egidio D'Angelo, Univ. of Pavia (Italy)
Francesco S. Pavone, European Lab. for Non-linear Spectroscopy, Univ. of Florence (Italy)
Univ. of Florence (Italy)


Published in SPIE Proceedings Vol. 7161:
Photonic Therapeutics and Diagnostics V
Henry Hirschberg; Brian Jet-Fei Wong; Kenton W. Gregory; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Guillermo J. Tearney; Justus F. R. Ilgner; Steen J. Madsen; Laura Marcu; Haishan Zeng, Editor(s)

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