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Proceedings Paper

Coupling confocal fluorescence microscopy and microfluidic device for single molecule detection
Author(s): Guoqing Shen; Baohe Chang; Bryan D. Dickerson; Xiaoxuan Li; Lloyd M. Davis
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Paper Abstract

The ability to accurately measure the mobility of particles at low concentrations in small volumes is very useful for a broad range of applications. The coupling of micro- and nano-fluidic devices and confocal microscopy offers an efficient and rapid technique for multiplexed single molecule detection and analysis. Microfluidic channels at micron and sub-micron scales were designed and fabricated on fused silica wafers. Fluorescence correlation spectroscopy and fluorescence lifetime were applied to measure and analyze the mobility of fluorescent species in micro-droplets, micro-channels, and nano-channels. The experimental results show

Paper Details

Date Published: 24 February 2009
PDF: 9 pages
Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070H (24 February 2009); doi: 10.1117/12.807962
Show Author Affiliations
Guoqing Shen, Luna Innovations Inc. (United States)
Baohe Chang, Luna Innovations Inc. (United States)
Bryan D. Dickerson, Luna Innovations Inc. (United States)
Xiaoxuan Li, The Univ. of Tennessee Space Institute (United States)
Lloyd M. Davis, The Univ. of Tennessee Space Institute (United States)

Published in SPIE Proceedings Vol. 7207:
Microfluidics, BioMEMS, and Medical Microsystems VII
Wanjun Wang, Editor(s)

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