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Proceedings Paper

Vibrational spectroscopy in biomedical science: bone
Author(s): Sonja Gamsjäger; R. Zoehrer; P. Roschger; P. Fratzl; K. Klaushofer; R. Mendelsohn; E. P. Paschalis
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Paper Abstract

Fourier transform infrared imaging (FTIR) and Raman Microspectroscopy are powerful tools for characterizing the distribution of different chemical moieties in heterogeneous materials. FTIR and Raman measurements have been adapted to assess the maturity of the mineral and the quality of the organic component (collagen and non-collagenous proteins) of the mineralized tissue in bone. Unique to the FTIRI analysis is the capability to provide the spatial distribution of two of the major collagen cross-links (pyridinoline, and dehydro-dihydroxylysinonorleucine) and through the study of normal and diseased bone, relate them to bone strength. These FTIR parameters have been validated based on analysis of model compounds. It is widely accepted that bone strength is determined by bone mass and bone quality. The latter is a multifactorial term encompassing the material and structural properties of bone, and one important aspect of the bone material properties is the organic matrix. The bone material properties can be defined by parameters of mineral and collagen, as determined by FTIR and Raman analysis. Considerably less attention has been directed at collagen, although there are several publications in the literature reporting altered collagen properties associated with fragile bone, in both animals and humans. Since bone is a heterogeneous tissue due to the remodeling process, microscopic areas may be carefully selected based on quantitative Backscattered Electron Imaging or histological staining, thus ensuring comparison of areas with similar metabolic activity and mineral content. In conclusion, FTIRI and Raman vibrational spectroscopy are proving to be powerful tools in bone-related medical research.

Paper Details

Date Published: 18 February 2009
PDF: 15 pages
Proc. SPIE 7166, Optics in Bone Biology and Diagnostics, 716602 (18 February 2009); doi: 10.1117/12.807914
Show Author Affiliations
Sonja Gamsjäger, Ludwig Boltzmann Institute of Osteology (Austria)
R. Zoehrer, Ludwig Boltzmann Institute of Osteology (Austria)
P. Roschger, Ludwig Boltzmann Institute of Osteology (Austria)
P. Fratzl, Max Planck Institute for Colloids and Interfaces (Germany)
K. Klaushofer, Ludwig Boltzmann Institute of Osteology (Austria)
R. Mendelsohn, Rutgers Univ. (United States)
E. P. Paschalis, Ludwig Boltzmann Institute of Osteology (Austria)

Published in SPIE Proceedings Vol. 7166:
Optics in Bone Biology and Diagnostics
Andreas Mandelis, Editor(s)

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