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Proceedings Paper

Fluorescence microscopy with a coded aperture snapshot spectral imager
Author(s): Christy A. Fernandez; Ashwin Wagadarikar; David J. Brady; Scott C. McCain; Tim Oliver
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Paper Abstract

This paper describes the application of a coded aperture snapshot spectral imager (CASSI) to fluorescence microscopy. CASSI records an interleaved spatially varying, spectrally filtered map of an object on a 2D focal plane. Convex optimization techniques combining least squares QR factorization with a total variance constraint are used to reconstruct a 3D data cube from a spectrally encoded 2D scene. CASSI records a 3D dataset at video rate - making it suitable for dynamic cellular imaging. We report on the reconstruction of fluorescent microspheres used in fluorescence microscopy applications and compare the results with images from a multi-spectral confocal system.

Paper Details

Date Published: 4 March 2009
PDF: 11 pages
Proc. SPIE 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, 71840Z (4 March 2009); doi: 10.1117/12.807863
Show Author Affiliations
Christy A. Fernandez, Duke Univ. (United States)
Ashwin Wagadarikar, Duke Univ. (United States)
David J. Brady, Duke Univ. (United States)
Scott C. McCain, Applied Quantum Technologies (United States)
Tim Oliver, Duke Univ. (United States)


Published in SPIE Proceedings Vol. 7184:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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