Share Email Print
cover

Proceedings Paper

A universal label-free biosensing platform based on opto-fluidic ring resonators
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Rapid and accurate detection of biomolecules is important for medical diagnosis, pharmaceuticals, homeland security, food quality control, and environmental protection. A simple, low cost and highly sensitive label-free optical biosensor based on opto-fluidic ring resonator (OFRR) has been developed that naturally integrates microfluidics with ring resonators. The OFRR employs a piece of fused silica capillary with a diameter around 100 micrometers. The circular cross section of the capillary forms the ring resonator and light repeatedly travels along the resonator circumference in the form of whispering gallery modes (WGMs) through total internal reflection. When the capillary wall is as thin as a couple of micrometers (< 4 μm), an evanescent field of the WGMs exists at the OFRR inner surface and interacts with the sample when it flows through the OFRR. In order to detect the target molecules with high specificity, the OFRR inner surface is functionalized with receptors, such as antibodies, peptide-displayed bacteriophage or oligonucleotide DNA probes. The WGM spectral position shifts when biomolecules bind to the OFRR inner surface and change the local refractive index, which provides quantitative and kinetic information about the biomolecule interaction near the OFRR inner surface. The OFRR has been successfully demonstrated for detection of various types of biomoelcuels. Here, we will first introduce the basic operation principle of the OFRR as a sensor and then application examples of the OFRR in the detection of proteins, disease biomarkers, virus, DNA molecules, and cells with high sensitivities will be presented.

Paper Details

Date Published: 19 February 2009
PDF: 13 pages
Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 71670I (19 February 2009); doi: 10.1117/12.807792
Show Author Affiliations
Hongying Zhu, Univ. of Missouri (United States)
Ian M. White, Univ. of Missouri (United States)
Jonathan D. Suter, Univ. of Missouri (United States)
John Gohring, Univ. of Missouri (United States)
Xudong Fan, Univ. of Missouri (United States)


Published in SPIE Proceedings Vol. 7167:
Frontiers in Pathogen Detection: From Nanosensors to Systems
Philippe M. Fauchet, Editor(s)

© SPIE. Terms of Use
Back to Top