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Proceedings Paper

Non-invasive measurements of hemoglobin + myoglobin, their oxygenation and NIR light pathlength in heart in vivo by diffuse reflectance spectroscopy
Author(s): Eugene Gussakovsky; Olga Jilkina; Yanmin Yang; Valery Kupriyanov
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Paper Abstract

The existing non-invasive optical methods of the hemoglobin (Hb) and myoglobin (Mb) estimation in cardiac tissues imply knowledge of the light pathlength (L) when various modifications of Lambert-Beer law for either spectrophotometry or light diffuse reflectance is applied. For Hb and/or Mb quantification in tissue, a few invasive (biochemical) approaches were applied. For L (differential pathlength factor; DPF) determination in tissue, special optical methods were used. No approaches have been proposed to simultaneously and non-invasively determine Hb/Mb and L in cardiac or other muscle tissues. In the present study, the first derivative of the NIR diffuse reflectance spectrum is shown to be effective in simultaneous determination of Hb+Mb concentration (in mM) and L (in mm) in cardiac tissue in vivo. The results showed that measured in a few minutes in a normal pig heart in vivo the total Hb+Mb concentration was 0.9-1.2 mM of heme, tissue oxygen saturation parameter (OSP) was approximately 65%, and DPF at 700-965 nm was of 2.7-2.8. At the experimental ischemia, total [Hb+Mb] decreased by 25%, OSP reduced to zero, while DPF did not change. These results correlated with the previously published. The method may be applied during open-heart surgery, heart studies ex vivo or to any muscle tissue to continuously and non-invasively monitor the [Hb+Mb] content and oxygenation as well as L, which may reflect the changes in tissue structure.

Paper Details

Date Published: 23 February 2009
PDF: 12 pages
Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 71612L (23 February 2009); doi: 10.1117/12.807715
Show Author Affiliations
Eugene Gussakovsky, National Research Council Canada (Canada)
Olga Jilkina, National Research Council Canada (Canada)
Univ. of Manitoba (Canada)
Yanmin Yang, National Research Council Canada (Canada)
Valery Kupriyanov, National Research Council Canada (Canada)
Univ. of Manitoba (Canada)


Published in SPIE Proceedings Vol. 7161:
Photonic Therapeutics and Diagnostics V
Henry Hirschberg; Steen J. Madsen; Brian Jet-Fei Wong; Justus F. R. Ilgner; Kenton W. Gregory; Guillermo J. Tearney; Laura Marcu; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Haishan Zeng, Editor(s)

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